我们测序克隆了7584条水稻（Oryza sativa）克隆的mRNA 序列，其中3712条已经上传在美国国家医学图书馆。
以下全长 cDNA 文库来源于在正常水培条件下生长三周的水稻根部。cDNA 群体经过标准化处理，并根据片段长度分为两个区间（0.5–2.0 kb 和 2.0–6.0 kb）后分别混合。所有 cDNA 均以 5′ 至 3′ 方向构建于修饰后的酵母表达载体启动子之后，使用 PmeI/NotI 克隆位点进行插入。每个文库在构建过程中均进行了严格的质量控制，并通过多种方法验证，包括用于检测全长和标准化效果的 Northern 杂交、PCR 扩增和限制性酶切分析，以及测序以检测平均插入片段长度和重组效率等。我们文库的一般特性可总结如下：
Following full-length cDNA libraries derived from roots of three-week-old rice plant growth under normal hydroponic conditions. cDNA populations were normalized, fractionated and pooled in two size ranges (0.5-2.0 kb and 2.0-6.0 kb). All cDNAs in the orientation of 5' to 3' end were constructed directly after a promoter of a modified year expression vector by using PmeI/NotI cloning sites. The quality of each library was stringently controlled during construction procedures and verified by combined approaches including Northern blotting for full-length and normalization test, PCR amplification and restriction digestion as well as sequencing for testing of average insert size and percentage recombination efficiency, etc. General specifications of our libraries can be summarized:
水稻根cDNA克隆（O. sativa，栽培型）：从全长cDNA文库中分离出以下具有GenBank登录号的克隆。cDNA已插入载体pYEXP7，用于在酵母（Sc）细胞中功能性表达水稻cDNA。
Rice root cDNA clones (O. sativa, japonica): following clones with GenBank acc. no. were isolated from the Full-Length cDNA Library. cDNA was inserted into the vector pYEXP7 for functional expression of the rice cDNA in yeast (Sc) cells.
在拟南芥、水稻等根细胞类型中特异性驱动基因表达的启动子。
Promoters driving a gene expression specifically in root-cell types of Arabidopsis, rice...
本服务将帮助客户通过酵母互补技术筛选我们的cDNA文库，或通过从cDNA文库进行分子克隆，分离/鉴定功能性植物（如水稻）基因。
This service will help customers to isolate/identify a functional plant (e.g. rice) gene(s) by either yeast complementation-based screening of our cDNA library or molecular cloning from the cDNA library.
在本服务中，我们将使用‘分裂泛素酵母双杂交系统’检测客户关注的蛋白-蛋白相互作用。
In this service we will apply the “Split-Ubiquitin Yeast Two Hybrid System” to test protein-protein interaction(s) of customer’s interest.
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Following full-length cDNA libraries derived from roots of three-week-old rice plant grown under normal hydroponic conditions. cDNA populations were normalized, fractionated and pooled in two size ranges (0.5-2.0 kb and 2.0-6.0 kb).
Rice root cDNA clones (O. sativa, japonica): following clones with GenBank acc. no. were isolated from the Full-Length cDNA Library. cDNA was inserted into the vector pYEXP7 for functional expression of the rice cDNA in yeast (Sc) cells.
在拟南芥、水稻等根细胞类型中特异性驱动基因表达的启动子.Promoters driving a gene expression specifically in root-cell types of Arabidopsis, rice...
This service will help customers to isolate/identify a functional plant (e.g. rice) gene(s) by either yeast complementation-based screening of our cDNA library or molecular cloning from the cDNA library.
In this service we will apply the “Split-Ubiquitin Yeast Two Hybrid System” to test protein-protein interaction(s) of customer’s interest.
Nullam ac rhoncus sapien, non gravida purus. Alinon elit imperdiet congue. Integer elit imperdiet congue. Integer ultricies sed ultricies sed ligula eget tempus.
